I don't think it's possible to refill those Winekeeper nitrogen canisters.
I have an older Winekeeper system that uses a standard nitrogen cylinder. It's good for a lot more bottles before you need to refill it, and the refill consists of a cylinder exchange at the local welding supply shop, and costs 1/3 less than a new Winekeeper canister. The original refillable nitrogen cylinder does cost about $40, though, but you soon get that investment back.
You can convert one of the disposable-canister Winekeeper systems to a refillable-cylinder system. I did this some years back with a disposable-cylinder Winekeeper that my brother had bought. The company that makes the Winekeeper (see
http://www.winkekeeper.com) also sells spare parts for the reusable-canister system. I don't remember the details, but the gist is that you buy a regulator that fits a standard refillable nitrogen cylinder and you just connect the plastic tube from your disposable-cylinder system to it. Call the tech support folks at Winekeeper and they can advise you as to what parts you need.
-Paul W.